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DNA extractions

JP Jeewan Pandey
DS Douglas C. Scheuring
JK Jeffrey W. Koym
JC Joseph Coombs
RN Richard G. Novy
AT Asunta L. Thompson
DH David G. Holm
DD David S. Douches
JJ J. Creighton Miller, Jr.
MV M. Isabel Vales
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Genomic DNA was extracted from 50 to 80 mg of fresh young potato leaves from tissue culture plantlets using the DNeasy Plant Pro Kit (Qiagen, Valencia, CA, USA). DNA quality was examined using 1% agarose gel 1X TBE (Tris–Borate and ethylenediaminetetraacetic acid) and staining with GelRed (Biotium Inc., CA, USA) using a V.U.V. transilluminator (Benchtop VUV Transilluminators, UVP). Quantification of DNA was performed in a spectrophotometer (Nano Drop, Thermo Scientific, Waltham, Massachusetts, USA). DNA concentration was verified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, SanDiego, CA) and samples with uniform DNA concentration (50 ng μL−1) were prepared.

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