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Luciferase reporter vectors were transfected into the Bel-7402 and SMMC-7721 cell lines along with the Renilla luciferase expression plasmid. Cells were incubated for 48 h and lysed with passive lysis buffer according to the instructions of a dual-luciferase system (Promega, WI). Signals were measured immediately using a microplate reader.
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