2.3. Cell Viability Assay

The cell density consisted of (1 × 10⁵ cells/well planted and cultured in 96-well plates. The cells were then cultured for 24 hours to allow for growth and adherence to the wall. Alloimperatorin was subsequently added at a concentration of 5, 25, 50, 100, 150, and 200 μM. The control group was grown in 0.1% DMSO. After 48 hours of treatment, 10 μl of CCK-8 solution was added and incubated at 37°C and 5% CO₂ for 1 hour. The OD values were detected at 450 nm with a microplate reader. The half-maximum inhibitory concentration (IC50) was calculated using Prism-GraphPad8.0 software. Cell viability (%) = [1–(ODcontrol−ODtreated)/(ODcontrol–ODblank)].