2.5. Construction of the Target-Pathway Network

Targets enriched in the pathways associated with hyperuricemia were ascertained, and the active ingredients acting on these targets were identified. The interaction among the active ingredients, targets, and pathways was established using nodes to represent the active ingredients, targets, and pathways and edges to represent the association between a certain target and a certain pathway or the action of an active ingredient on a certain target. The file data were imported into Cytoscape 3.8.0 to construct an “active ingredient-target-pathway” network, and the topological attributes of the nodes in this network were analyzed to clarify the material basis and multi-ingredient, multitarget, and multipathway molecular mechanisms for the treatment of hyperuricemia with Plantaginis Herba.