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Alizarin Red S Staining Assay

YZ Yibo Zhang
YL Yawen Li
WL Wei Liao
WP Wenzao Peng
JQ Jianghui Qin
DC Dongyang Chen
LZ Liming Zheng
WY Wenjin Yan
LL Lan Li
ZG Zhirui Guo
PW Peng Wang
QJ Qing Jiang
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After osteogenic induction for 21 days, MC3T3-E1 cells, rBMSCs, and PDLPs treated with citrate-stabilized AuNRs of various aspect-ratio were treated with 5% alizarin red S (ARS; Sigma) staining solution for 5 min. Subsequently, the cells were washed and examined, and images captured by an inverted optical microscope (Olympus IMT-2, Tokyo, Japan), and the formation of mineralized matrix nodules was quantified by measuring the absorbance at 562 nm after incubation with 10% (w/v) cetylpyridinium chloride (Sigma-Aldrich) for 2 h.

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