Carry-over contamination rate mainly reflects the degree of contamination between samples. By measuring the high concentration sample 3 times (H1, H2, H3) and low concentration sample 3 times (L1, L2, L3), the carry-over contamination rate was calculated: carry-over contamination rate (%) = (L1 - L3)/(H3 - L3) ∗ 100%. When the carry-over contamination rate was not higher than 3%, the carry-over contamination rate would be accepted.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.