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Ovary dissection and fixation and durcupan sectioning
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Adult Gryllus females were paralysed using CO2. Ovaries were dissected quickly in ice-cold PBS with fine forceps and were fixed for overnight in a 5% formaldehyde/PBST solution (Lynch et al., 2010). Durcupan sections on Gb-pipe-stained oocytes were performed as described in Pechmann et al., 2017 with minor modifications.
Copyright and License information: ©2021, Pechmann et al
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