According to the EP5-A guideline published by CLSI,[11] precision was evaluated by calculating the coefficients of variations (CVs) of intra-assay and inter-assay. The experiments were performed using 2 concentrations (low concentration and concentration) of serum samples, which were analyzed twenty times, respectively. CV (%) = (Standard deviation/average) ∗ 100%. When the CV was no more than 10.0%, the precision would be accepted.
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