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Otsu binarization (thresholding)
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Otsu’s method [17] is used for automatic thresholding of binary images in order to separate two object classes in microscopy datasets—specifically fluorescently labelled nuclei from its background [15,16]. The algorithm determines the threshold value that minimizes the intra-class variance, defined as a weighted sum of variances of these two classes:
Copyright and License information: ©2021 Englbrecht et al
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