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The TdT‐mediated dUTP Nick‐End Labelling (TUNEL) technique was used to evaluate neuronal apoptosis. The In Situ Cell Death Detection Kit (Roche, Switzerland, Germany, 11 684 795 960) was used according to the instruction. The nuclei were stained with DAPI, and fluorescent microscopy (Olympus, Tokyo, Japan) was used to evaluate neuronal apoptosis.
Copyright and License information: ©2021 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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