2.10. Flow cytometry analysis of cell cycle and cell apoptosis

To analyse the cell cycle, cells were transfected with RCC1‐siRNA and NC‐siRNA, and digested and washed twice with PBS and fixed with 75% precooled ethanol. Subsequently, the cell cycle analysis was performed by flow cytometry (BD Biosciences, USA).

For cell cycle apoptosis, after the same transfection for 48 hours, the cells were collected with PBS and centrifuged 3 times. Apoptosis was then detected by Annexin V‐FITC APOPTOSIS KIT (Ebioscience, USA) and analysed by Flow cytometry according to the instructions (BD Biosciences, USA).