The upper chamber was fixed with 100 µL serum‐free suspension, and the bottom chamber was fixed with 600 µL DMEM with 10% FBS. Twenty‐ four hours after incubation, cells (1 × 103 cells for each group) were treated with crystal violet staining. Penetrating cells were calculated with 5 randomly picked fields, and calculation repeated three times. Experiment was repeated three times.
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