Enzyme-linked immunosorbent (ELISA)

JZ Junshuang Zhao
YJ Yang Jiang
HZ Haiying Zhang
JZ Jinpeng Zhou
LC Lian Chen
HL Hao Li
JX Jinkun Xu
GZ Guoqing Zhang
ZJ Zhitao Jing
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ELISA was performed as previously described [27]. The Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab46027) was used to detect the concentrations of IL6 in the media supernatant of GSCs. All ELISA readings were normalized to the protein concentration in the control groups.

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