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Tissue dissection for Western blotting and quantitative PCR (qPCR)

PV Philip Vassilev
AP Andrea Haree Pantoja-Urban
MG Michel Giroux
DN Dominique Nouel
GH Giovanni Hernandez
TO Taylor Orsini
CF Cecilia Flores
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Mice from the control, susceptible and resilient groups were euthanized by decapitation 7 d after the SIT. Brains were removed and flash frozen with 2-methylbutane chilled in dry ice. Bilateral punches of the pregenual mPFC [including prelimbic (PrL) and infralimbic (IL) subregions], the NAcc, and the ventral tegmental area (VTA) were taken from 1 mm-thick coronal sections, starting from plate 14 of the mouse brain atlas (Paxinos and Franklin, 2019) for mPFC and NAcc, and plate 55 for VTA.

Copyright and License information:  ©2021 Vassilev et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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