Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Worm strains and maintenance

RP Rosemary N. Plagens
IM Isiah Mossiah
KG Karen S. Kim Guisbert
EG Eric Guisbert
request Request a Protocol
ask Ask a question
Favorite

The following Caenorhabditis elegans strains were used in this study: Bristol strain N2, RT130(pwIs23[YP170::GFP]), RT362(rme-4(b1001);pwIs23[YP170::GFP]), GS1912(arIs37 [myo-3p::ssGFP + dpy-20(+)] I), and EQ140(iqIs37[pAH76(hsf-1p::myc-hsf-1)+pRF4(rol-6p::rol-6(su1006)]). Unless otherwise stated, worms were maintained at 20 °C. Worm populations were synchronized for experiments by allowing day 2 adult hermaphrodites to lay eggs for 1–1.5 h. The synchronized egg populations were then raised at 20 °C until day one of adulthood, after the onset of egg laying (~ 72 h post-synchronization for the N2 strain). For all heat stress (HS) experiments, plates of day 1 adults were transferred to a 28 °C dry incubator (HS worms) for the specified time course.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A