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After an overnight fast, peripheral blood was collected and PBMCs were isolated by Ficol separation. Cells were lysed with trizol lysis buffer and lysates were stored at − 80 °C for further analysis. Total RNA was extracted from PBMCs using the miRNEasy Kit (QIAGEN, Hilden, Germany). The integrity of RNA samples was assessed using Agilent 2100 yielding high RIN between 9.1 and 10.

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