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High performance liquid chromatography
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Media and tissue analyses were performed with a Waters 2690 HPLC instrument, using a Zorbax RX‐C18 250 mm × 4.6 mm column and a mobile phase composed of acetonitrile (ACN)/water: CP and BV (45% ACN in water for 15 min, increasing to 100% ACN after 16 min) and HV (40% ACN in water for 20 min, increasing to 100% ACN after 30 min), all at 1 ml min–1. Emission of UV light was detected using a Waters 486 UV detector at 240 nm (for CP and BV) and 244 nm (for HV) and the concentrations of these corticosteroids was calculated from standard calibration curves.
Copyright and License information: ©2020 The Authors. s published by John Wiley & Sons Ltd on behalf of Scandinavian Division of the International Association for Dental Research
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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