Alcian blue staining

ZZ Zemin Zhu
HX Hongyuan Xing
RT Ruofu Tang
SQ Shengjun Qian
SH Shaoqi He
QH Qiang Hu
NZ Ning Zhang
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The ECM deposit was also determined by the Alcian blue staining. Briefly, after differentiation, cells were washed with PBS, then fixed with 4% paraformaldehyde for 15 min. The cells were stained using Alcian blue solution for 30 min and washed twice with PBS before observation under microscope.

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