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Cells were seeded in 6-well plate at a density of 2 × 105/well and allowed to adhere overnight. Treatment and incubation period were performed as required. Cell apoptosis was determined by Annexin V/PI labeling according to the manufacturer’s protocol (Invitrogen). The early and late apoptotic cells were detected using a flow cytometry (BD FACS CantoTM, BD Biosciences, San Jose, CA, United States) based on the Annexin V and PI staining.
Copyright and License information: ©2017 Zhong, Yu, Wang, Qiang, Wang, Zhang, Yu, Cui, Wu, Li and Wang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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