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Changes in the physiological parameters of immunized raccoon dogs upon virus challenge

LX Linya Xia
GL Guoliang Luo
MW Mingjie Wu
LW Lei Wang
NZ Ning Zhang
CW Congmei Wu
YY Yuhe Yin
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Twenty-five raccoon dogs were divided into 5 groups (n = 5). Groups A, B, C were immunized with either 10 μg, 50 μg, or 100 μg RDPV VLP treated with 20 mg/mL Al(OH)3 gel. Additionally, group D was vaccinated with 100 μL of experimentally inactivated RDPV vaccine (HA titer 1:211), while group E was vaccinated with 100 μL of PBS. Raccoon dogs described above were struck with RDPV RPSN virus (HA titer 1:211) containing 10 mL oral MEM medium (Gibco, USA) at 21 dpi. The diet, mental health and the stool samples of raccoon dogs were evaluated. Stool samples were collected and tested with canine parvovirus detection kit (Sinohp, China). Additionally, 1 g stool samples were mixed evenly with 1 mL PBS, and the mixture was centrifuged at 10,000g/min for 5 min. The HA titer was estimated from the collected supernatant. Blood samples were collected and analyzed with a Blood Chemistry Analyzer (ABAXIS, USA). The extracted serum was used to detect HI titer. Stool and blood samples from every group were collected and labelled at 8 o'clock every morning.

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