Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Day 4/6/8/10/12: Selection of stably integrated heterozygous KCNH2 clones
Ask a
question
Favorite
Replace the culture media in each T25 flask with BH-DMEM (DMEM supplemented with 10% FBS, 10 ug/mL Blasticidin and 200 ug/mL Hygromycin) (Thermofisher, cat. # 10687010) at 2-day intervals.
Copyright and License information: ©2021 The Author(s) 2021. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
