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Metabolite Extraction

YL Yuting Liu
MW Mutsumi Watanabe
SY Sayuri Yasukawa
YK Yuriko Kawamura
CA Chaiwat Aneklaphakij
AF Alisdair R. Fernie
TT Takayuki Tohge
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Metabolite extraction was conducted as previously described (Tohge and Fernie, 2010). About 15 mg of frozen sample was weighed. Extraction buffer i.e., 80% methanol (5 μg/ml isovitexin as an internal standard) was added at a ratio of 1 mg F.W. sample powder: 10 μl extraction buffer. The mixture was vortexed to mix thoroughly and centrifuged at 14,000 rpm at 4°C for 10 min, then supernatant was transferred into new 1.5 ml tube and centrifuged once more, the final obtained supernatant was transferred into vials for liquid chromatography-mass spectrometry (LC-MS) and high performance liquid chromatography-photodiode array detector (HPLC-PDA).

Copyright and License information:  ©2021 Liu, Watanabe, Yasukawa, Kawamura, Aneklaphakij, Fernie and Tohge.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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