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The IAA production activity was tested using TSB (tryptone soy broth) agar and incubated at 28–30°C for 2–5 days. Approximately 1 ml of Salkowsky solution was dropped on top of the growing colonies and then incubated in a dark condition for 1 hour. A positive result was noted by a color change to pink indicating that these isolates were able to produce IAA (18).
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