Exosome extraction using the exoEasy Maxi Kit

Cells were centrifuged at 16,000 g for 10 min. The exosomes were isolated using the exoEasy Maxi Kit (QIAGEN®, Germany). The suspension was mixed with XBP buffers and transferred to an affinity spin column, which was centrifuged at 600 g for 50 s. XWP buffer (3.5 ml) was added, and the column was centrifuged for 5 min at 5000 g. The eluate (700 μl) was added to a QIAzol membrane and centrifuged at 5000 g for 5 min.