Real-time unlabeled cell analysis technique

MCF-7 and A2780 cell proliferation was analyzed with real-time cell analysis (RTCA). MCF-7 and A2780 cells, used as targeted cells, were observed every 5 minutes after incubation using the xCELLigence system E-Plate. Cell index (CI) values were recorded and proliferation was observed continuously for 120 hours. Cell proliferation capacity was determined by calculating the difference in CI compared to the controls and analyzing the slope of the curve between 0 and 120 hours (26).