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Ovarian cancer cell migration was evaluated by the wound healing assay. Cells were cultivated in 6-well plates after 48 h of transfection with STK17B siRNA. 90% confluence was obtained before transfection. A clean line in the middle of 6-well plates by 200-μL sterilization pet tips, then washed the floating cells with PBS. Finally, the travel distances were taken pictures at 0 and 24 h in order to estimate the ability of cell wound healing.
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