Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

In situ Hybridization

XH Xiaolan He
PL Peng Liu
XZ Xiao Zhang
ZJ Zhenhua Jiang
NG Nan Gu
QW Qun Wang
YL Yan Lu
request Request a Protocol
ask Ask a question
Favorite

Methods for in situ hybridization (ISH) which were related to vesicular glutamate transporter 2 (Vlgut2) have been described in previous articles.10,18,19 Briefly, sections from three GlyT2-tdTomato male mice were applied in this procedure. For the acquisition of tdTomato fluorescent images, slices were captured under a confocal scanning microscope installed with laser (Olympus FV1200, Japan). In situ signals of Vglut2 were obtained under transluminescent light and pseudo-green fluorescent color. The overlay between red fluorescence positive images and Vglut2 (green) images was conducted by Photoshop software (USA)

Copyright and License information:  ©2021 He et al.
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A