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4.7. Proteasome Activity Assay
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The proteasomal activity was assessed in cytosolic cell extracts by measuring the hydrolysis of fluorogenic peptide substrates as described [40]. Z-LLE-AMC and Suc-LLVY-AMC (Biomol, Hamburg, Germany) were used for the measurement of caspase-like and chymotrypsin-like activities, respectively. Cellular extracts were incubated with the substrates in 96-well plates, and the release of the fluorescent free AMC group was measured by the infinite 200 multifunctional microplate reader (TECAN, Mannedorf, Switzerland).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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