4.9. Determination of the Bleeding Time to Investigate Hemostasis in Transgenic Mice

Mice at the age of 10–12 weeks were anesthetized with Ketamin (100 mG/kg) and Xylazin (5 mG/kg) i.p. and placed in prone position. A transverse incision was made with a scalpel at a position where the diameter of the tail is 2.25–2.5 mm using a gauge to cut a defined size of the tail tip. The tail was immediately immersed in a 15 mL Falcon tube containing pre-warmed PBS in a water bath to 37 °C and bleeding was continuously monitored. Each animal was monitored for 20 min even if bleeding stopped in order to detect any re-bleeding events. The time from the incision to the cessation of bleeding was recorded using a stop clock.