4.6. Platelet Dense Granule Secretion Assay

For quantification of ATP release from platelets dense granules, platelets in buffer were incubated with different inhibitors or DPBS for 30 min, respectively. AsPC-1, Capan-2, and MIA PaCa-2 cells were detached with EDTA and suspended in DPBS. After incubation of platelets (4 × 10⁸ Plts/mL) with 1 × 10⁴ tumor cells/mL for 20 min, ATP concentration was quantified by luminescence measurement using a luciferin-based ATP-Determination Kit (Thermo Fisher Scientific, Waltham, MA, USA) and a FLUOstar Optima plate reader (BMG Labtech).