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cAMP analysis was performed as described previously [10]. Primary cortical cells were cultured and treated on DIV3 with synaptamide or A8 for 10 min to determine cAMP levels using a cyclic AMP XP assay kit (Cell Signaling Technology, Danvers, MA, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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