4.3. Platelet Aggregation-Based Selectivity Assays

Aggregations were performed in platelet-rich-plasma (PRP) or washed platelets previously warmed at 37 °C for 4 min without stirring and for 1 min with constant stirring at 1200 rpm in a Chrono-log^® 490-X aggregometer (Chrono-log Corporation, Havertown, PA, USA). After that, PRP or washed platelets were stimulated with the selected agonists for 5 min. The agonists used in the assay were: CRP-XL (Cambcol Laboratories Ltd., Cambridgeshire, UK), Horm^® collagen (Takeda Austria GmbH, Linz, Austria), rhodocytin (provided by Johannes A. Eble, from the Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Münster, Germany), thrombin receptor activating peptide (TRAP) (Tocris Bioscience, Bristol, UK), and thrombin (Sigma-Aldrich, Saint Louis, MO, USA).