4.5. Clonogenic Survival Assays

Clonogenic survival assays were done as described [17]. Twenty-four hours post seeding, cells were washed with PBS, treated with varying doses of cisplatin and left to grow in the presence of cisplatin for the entirety of the experiments. Ten days post treatment, colonies were fixed and stained with a solution containing 50% methanol, 7% acetic acid and 0.1% Coomasie blue for 10 min at room temperature. Colonies consisting of more than 32 cells were manually scored on a microscope (Leica EZ4, Concord, ON, Canada).