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4.4. Determination of Amicoumacins by HPLC-MS

JY Jie Yang
QZ Qingzheng Zhu
FX Feng Xu
MY Ming Yang
HD Hechao Du
XB Xiaoying Bian
ZL Zhaoxin Lu
YL Yingjian Lu
FL Fengxia Lu
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For liquid chromatography high-resolution mass spectrometry (LC-HRMS) analysis, an UltiMate 3000 HPLC system (Dionex, Sunnyvale, CA, USA) equipped with a C18 column (5 µm, 4.6 × 250 mm i.d., Agilent Technologies, Palo Alto, CA, USA) was used. The spray voltage was set to 4.0 kV, and the heated transfer capillary temperature was set to 350 °C. High-resolution mass spectrometry analysis was performed with a Thermo Finnigan Surveyor-LCQ DECA XP Plus (Thermo Electron Corporation, San Jose, CA, USA) equipped with an electrospray ionization (ESI) source.

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