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Fluorescence recovery after photobleaching (FRAP)

MZ Ming Zhao
YY Yu Yu
LS Li-Ming Sun
JX Jia-Qing Xing
TL Tingting Li
YZ Yunkai Zhu
MW Miao Wang
YY Yin Yu
WX Wen Xue
TX Tian Xia
HC Hong Cai
QH Qiu-Ying Han
XY Xiaoyao Yin
WL Wei-Hua Li
AL Ai-Ling Li
JC Jiuwei Cui
ZY Zhenghong Yuan
RZ Rong Zhang
TZ Tao Zhou
XZ Xue-Min Zhang
TL Tao Li
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Recombinant mEGFP-tagged N proteins were used to performed FRAP assays in vitro. Selected regions were bleached with a 488-nm laser pulse. The fluorescence intensity was collected every 1 s and normalized to the intensity before bleaching. For in vivo FRAP assays, H1299 cells were seeded on the glass bottom cell culture dishes and treated with 100 ng ml−1 Dox for the inducible expression of N-mEGFP. After 12-h Dox treatment, the cells were transfected with 1 μg ml−1 poly(I:C) for another 6 h. FRAP assays were performed with 488-nm laser pulse and the fluorescence intensity was collected every 0.5 s in vivo and normalized to the intensity before bleaching.

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