Assimilated energy and assimilation efficiency was assessed using the C200 Oxygen Bomb Calorimeter (IKA, Staufen, Germany). Food consumption and feces production were measured/collected over 7 days during continuous daily peripheral (s.c.) acyl-GIP treatment. Feces and food were dried for several days at 65 °C before measuring food/fecal energy content for assessment of assimilated energy (KJ/g food).
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