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TUNEL staining, flow cytometry, and caspase 3/7 activation assays were used to measure cellular apoptosis. For TUNEL staining, the DeadEnd™ Fluorometric TUNEL System (Promega, CA, USA) was used on the basis of the manufacturer's instructions. The Caspase-Glo® 3/7 Assay Kit (Promega) was used to assess caspase 3/7 activation. Human NP cell apoptosis was evaluated by flow cytometric analysis (Gallios, Beckman Coulter) of TUNEL/DAPI dual-staining (Invitrogen, CA, USA) based on the manufacturer's instructions.
Copyright and License information: ©2021 Zhao Guo et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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