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Extraction of Long-Chain Acyl-CoAs from Heart Tissues.

SS Sushabhan Sadhukhan
XL Xiaojing Liu
DR Dongryeol Ryu
ON Ornella D. Nelson
JS John A. Stupinski
ZL Zhi Li
WC Wei Chen
SZ Sheng Zhang
RW Robert S. Weiss
JL Jason W. Locasale
JA Johan Auwerx
HL Hening Lin
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All of the extraction solutions and solvents used for the CoA extraction were precooled on ice. Powder frozen heart tissues (∼20 mg) were homogenized with 0.5 mL of methanol/water 4:1 using an electronic mixer for 30 s on ice. The tissue homogenates were centrifuged at 20,000 × g for 15 min at 4 °C. The clear supernatant was dried in a SpeedVac. The dried residue was stored at −80 °C until LC-MS analysis.

Copyright and License information:  ©2016

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