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Transfection of miRNA Mimic or Inhibitor
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Gain or loss of miRNA function was achieved by transfection of 10 nM miRNA mimics or 100 nM inhibitors with Lipofectamine 3000 (Invitrogen, U.S.A.). The sequence information of mimics and inhibitors is shown in Supplementary Table 3. SW982 and RASF cells (1.5×105 cells/ml) were seeded in 6-well plates overnight before transfection. After transfection for 48h, RNA and protein were harvested in the same batch during each individual cell experiment.
Copyright and License information: ©2021 Wang, Xu, Guo, Cai, Ren, Zhu, Geng, Meng, Jiang and Lu
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