Curcumin (Sigma-Aldrich, St. Louis, MO) stock solutions were prepared using DMSO and ethanol as solvents. Subsequent dilutions were made from this stock solution in DMEM. The final concentrations of DMSO and ethanol did not exceed 0.04 % and 0.6%, respectively, and cell viability was not affected at these concentrations (data not shown).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.