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PICRUSt approach was used to evaluate the functional potential of the different microbiotypes27. The full-study ASV Table was converted to BIOM format using the biomformat v1.12.0 R package28 and was processed with PICRUSt229. EC number, KO metagenomes, as well as MetaCyc pathway abundances were predicted. Differences in MetaCyc pathway abundances between microbiotypes were further analyzed using the DESeq2 v1.24.0 R package30. A p-value of < 0.05 after Bonferroni’s multiple test correction was considered statistically significant.
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