2.1. Experimental Mice

C57BL/6 mice (20-30 g, male) from the Wenzhou Medical University Animal Center (authorization number SCXK (ZJ) 2015-0001) were nurtured under normal conditions (humidity: 50 ± 5%; temperature: 23 ± 2°C) on a light/dark cycle for 12 hours, and the mice were allowed to eat and drink freely. Surgery, treatment, and perioperative care strictly adhered to the “Guide to the Care and Use of Laboratory Animals of the Chinese Institute of Health.” All operations involving mice were approved by the Animal Research Committee of Wenzhou Medical University (wydw2017-0022).