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Adapter ligation

AW Amelia D. Wallace
TS Thomas A. Sasani
JS Jordan Swanier
BG Brooke L. Gates
JG Jeff Greenland
BP Brent S. Pedersen
KV Katherine E. Varley
AQ Aaron R. Quinlan
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An adapter ligation mix was prepared from the LSK-109 Ligation Sequencing Kit by combining 25μL Ligation Buffer, 5μL Quick T4 Ligase (NEB E6057), 5μL Adapter Mix, and 13μL nuclease-free water. The mixture was added to the previous reaction for a total volume of 100uL and incubated for 10 minutes on a hula mixer at room temperature. A clean-up step was then performed using 0.3X AmpureXP magnetic beads (Beckman Coulter A63881) and washed twice with 200μL of Short Fragment Buffer (ONT SQK-LSK109). The final library was eluted in 16.6μL of Elution Buffer and 15.8μL retained.

Copyright and License information:  ©2021 Wallace et al ©2021 Alfred S LewinThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Alfred S LewinThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Alfred S LewinThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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