Radioimmunoassay analysis of progesterone

KH Katherine M. Halloran
EH Emily C. Hoskins
CS Claire Stenhouse
RM Robyn M. Moses
KD Kathrin A. Dunlap
MS M. Carey Satterfield
HS Heewon Seo
GJ Gregory A. Johnson
GW Guoyao Wu
FB Fuller W. Bazer
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Maternal blood samples were collected immediately prior to euthanasia of ewes and stored on ice until processing. Plasma was collected following centrifugation (8000 × g for 7 min) and stored at −20 °C until analyzed. Concentrations of progesterone in maternal plasma were determined as previously described [34] using a Progesterone Coated Tube Radioimmunoassay Kit (07-270102, MP Diagnostics) validated with P4 in ovine plasma.

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