Maternal blood samples were collected immediately prior to euthanasia of ewes and stored on ice until processing. Plasma was collected following centrifugation (8000 × g for 7 min) and stored at −20 °C until analyzed. Concentrations of progesterone in maternal plasma were determined as previously described [34] using a Progesterone Coated Tube Radioimmunoassay Kit (07-270102, MP Diagnostics) validated with P4 in ovine plasma.
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