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Mitochondrial complex I activity was measured with the Complex I Activity Assay kit (cat. no. ab109721; Abcam) according to the manufacturers' instructions. The extracted cell proteins were loaded onto 96-well plates and incubated with mitochondrial complex I antibody for 3 h at room temperature. Each experiment was performed in five parallel wells and the mean optical density (OD 450 nm) values were measured using the Nano Drop spectrophotometer.
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