2.7. Western blotting

VP Vina Putra
AH Amy J. Hulme
AT Andrew E. Tee
JS Jane Q.J. Sun
BA Bernard Atmadibrata
NH Nicholas Ho
JC Jingwei Chen
JG Jixuan Gao
MN Murray D. Norris
MH Michelle Haber
MK Maria Kavallaris
MH Michelle J. Henderson
JM Joshua McCarroll
TT Toby Trahair
TL Tao Liu
PL Pei Y. Liu
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Western blotting was performed as previously described [21]. The primary antibodies used were as follows: rabbit anti‐DDX21 (1 : 500; Catalogue number A300‐629A; Bethyl Laboratories, Montgomery, TX, USA), rabbit anti‐CEP55 (1 : 500; Catalogue number 81693S; Cell Signaling Technology, Danvers, MA, USA) or mouse anti‐N‐Myc (1 : 1000; Catalogue number sc‐53993; Santa Cruz Biotechnology). The secondary antibodies used were as follows: goat anti‐rabbit (Catalogue number 12‐348) or goat anti‐mouse (Catalogue number 12‐349) (1 : 10 000; both from MERCK, Burlington, MA, USA). The anti‐actin antibody (1 : 15 000; Catalogue number A3853; Sigma) was used as loading control.

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