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t-test calculator was used for group comparisons of experimental myocardial infarction (MI) model rats. Two-tailed unpaired t-test was used to compare each two-group combination with significance at p < 0.05. The number of independent animals used for each group are described in the legend to Fig. 4. With limited plasma availability from each animal, measurement of rat plasma ANKRD1 was performed in duplicates in one ELISA assay. Intra-assay CV of all plasma ANKRD1 reported are less than 12%.
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