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Reagents and cell lines

JZ Jun-Yi Zheng
CW Chen-Yu Wang
CG Chuan Gao
QX Qiong Xiao
CH Cheng-Wei Huang
MW Min Wu
LL Lian-Yun Li
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Antibodies recognizing β-Actin (Abclonal, AC026, RRID: AB_2768234), H3 (abcam, ab1791, RRID: AB_302613), H3K27ac (abcam, ab4729, RRID: AB_2118291), H3K4me1 (Cell Signaling Technology, #5326, RRID: AB_10695148), p53 (Santa Cruz, sc-126, RRID: AB_628082), p21 (Cell Signaling Technology, #2947, RRID: AB_823586), MDM2 (abcam, ab3110, RRID: AB_303518), PUMA (Cell Signaling Technology, #4976, RRID: AB_2064551), MLL3(Merck ABE1851) and TNS3 (Abclonal A7991, RRID: AB_2772674) were purchased from indicated vendors. PCR primers were custom synthesized by TSINGKE and siRNAs by GenePharma. Nutlin-3A (S8059) was purchased from Selleck.

MDA-MB-231 and HeLa cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco) at 37 °C with 5% CO2, and U2OS and 769-P cells in RPMI 1640 (Gibco). Both mediums were supplemented with 10% FBS (Biological Industries) and 1% penicillin-streptomycin solution (HyClone). All cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences.

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