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Mix ATP6V0D2, Notch1, and BECN1 siRNA (Sangon Biotech, Shanghai, China) with mannose-conjugated polymer (polyplus-transfection SA, Illkirch, France) according to the manufacturer's instructions, injected through tail vein 4 h before performing IR surgery on mice (siRNA 2 mg/kg) to achieve knockdown in liver macrophages.
Copyright and License information: ©2021 Ziyi Wang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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