Animal model

The animal experiment was approved by the Experimental Animal Care and Use Committee of the Cancer Hospital of China Medical University. BALB/c mice were used to create a xenograft mouse model of the CT26 colon cancer cell line. All mice were 5-week-old females weighing 19–21 g (Animal Center of the Medical College of Beijing University, Beijing, China). All mice were maintained in a specific pathogen-free environment with free access to clean water and food. A total 5×10⁶ CT26 cells were injected into the flank of each mouse subcutaneously. One week later, mice were assigned to 3 groups (10 per group) to accept different treatment: Oxa, Oxa plus infliximab, or saline. Tumor volume was measured every week and survival status was recorded every day. Tumor volume was calculated following the formula: tumor volume=width²×length×π/6.